update range to 10000 bp
locus tag: FAM19471-i1-1.1_001330 genome: FAM19471-i1-1.1
species: Limosilactobacillus fermentum taxid: 1613

Annotations

Type Name Description Pathways
KEGG reaction
 R04463 5'-Phosphoribosylformylglycinamide:L-glutamine amido-ligase (ADP-forming); ATP + 5'-Phosphoribosyl-N-formylglycinamide + L-Glutamine + H2O <=> ADP + Orthophosphate + 2-(Formamido)-N1-(5'-phosphoribosyl)acetamidine + L-Glutamate kornec00230kornec01100kornec01110
Gene Code
 purL
Gene Product
 phosphoribosylformylglycinamidine synthase subunit PurL
Ortholog
 N0.HOG0001441 phosphoribosylformylglycinamidine synthase subunit PurL
KEGG gene
 K01952 PFAS, purL; phosphoribosylformylglycinamidine synthase [EC:6.3.5.3] kornec00230kornec01100kornec01110
Gene Ontology
 GO:1901576 organic substance biosynthetic process
Gene Ontology
 GO:1901566 organonitrogen compound biosynthetic process
Gene Ontology
 GO:1901564 organonitrogen compound metabolic process
Gene Ontology
 GO:1901363 heterocyclic compound binding
Gene Ontology
 GO:1901362 organic cyclic compound biosynthetic process
Gene Ontology
 GO:1901360 organic cyclic compound metabolic process
Gene Ontology
 GO:1901293 nucleoside phosphate biosynthetic process
Gene Ontology
 GO:1901265 nucleoside phosphate binding
Gene Ontology
 GO:1901137 carbohydrate derivative biosynthetic process
Gene Ontology
 GO:1901135 carbohydrate derivative metabolic process
Gene Ontology
 GO:0097367 carbohydrate derivative binding
Gene Ontology
 GO:0097159 organic cyclic compound binding
Gene Ontology
 GO:0090407 organophosphate biosynthetic process
Gene Ontology
 GO:0072522 purine-containing compound biosynthetic process
Gene Ontology
 GO:0072521 purine-containing compound metabolic process
Gene Ontology
 GO:0071704 organic substance metabolic process
Gene Ontology
 GO:0055086 nucleobase-containing small molecule metabolic process
Gene Ontology
 GO:0046483 heterocycle metabolic process
Gene Ontology
 GO:0046390 ribose phosphate biosynthetic process
Gene Ontology
 GO:0044281 small molecule metabolic process
Gene Ontology
 GO:0044271 cellular nitrogen compound biosynthetic process
Gene Ontology
 GO:0044249 cellular biosynthetic process
Gene Ontology
 GO:0044238 primary metabolic process
Gene Ontology
 GO:0044237 cellular metabolic process
Gene Ontology
 GO:0043168 anion binding
Gene Ontology
 GO:0043167 ion binding
Gene Ontology
 GO:0036094 small molecule binding
Gene Ontology
 GO:0035639 purine ribonucleoside triphosphate binding
Gene Ontology
 GO:0034654 nucleobase-containing compound biosynthetic process
Gene Ontology
 GO:0034641 cellular nitrogen compound metabolic process
Gene Ontology
 GO:0032559 adenyl ribonucleotide binding
Gene Ontology
 GO:0032555 purine ribonucleotide binding
Gene Ontology
 GO:0032553 ribonucleotide binding
Gene Ontology
 GO:0030554 adenyl nucleotide binding
Gene Ontology
 GO:0019693 ribose phosphate metabolic process
Gene Ontology
 GO:0019637 organophosphate metabolic process
Gene Ontology
 GO:0019438 aromatic compound biosynthetic process
Gene Ontology
 GO:0018130 heterocycle biosynthetic process
Gene Ontology
 GO:0017076 purine nucleotide binding
Gene Ontology
 GO:0016884 carbon-nitrogen ligase activity, with glutamine as amido-N-donor
Gene Ontology
 GO:0016879 ligase activity, forming carbon-nitrogen bonds
Gene Ontology
 GO:0016874 ligase activity
Gene Ontology
 GO:0009987 cellular process
Gene Ontology
 GO:0009260 ribonucleotide biosynthetic process
Gene Ontology
 GO:0009259 ribonucleotide metabolic process
Gene Ontology
 GO:0009165 nucleotide biosynthetic process
Gene Ontology
 GO:0009152 purine ribonucleotide biosynthetic process
Gene Ontology
 GO:0009150 purine ribonucleotide metabolic process
Gene Ontology
 GO:0009117 nucleotide metabolic process
Gene Ontology
 GO:0009058 biosynthetic process
Gene Ontology
 GO:0008152 metabolic process
Gene Ontology
 GO:0008150 biological_process
Gene Ontology
 GO:0008144 obsolete drug binding
Gene Ontology
 GO:0006807 nitrogen compound metabolic process
Gene Ontology
 GO:0006796 phosphate-containing compound metabolic process
Gene Ontology
 GO:0006793 phosphorus metabolic process
Gene Ontology
 GO:0006753 nucleoside phosphate metabolic process
Gene Ontology
 GO:0006725 cellular aromatic compound metabolic process
Gene Ontology
 GO:0006164 purine nucleotide biosynthetic process
Gene Ontology
 GO:0006163 purine nucleotide metabolic process
Gene Ontology
 GO:0006139 nucleobase-containing compound metabolic process
Gene Ontology
 GO:0005524 ATP binding
Gene Ontology
 GO:0005488 binding
Gene Ontology
 GO:0004642 phosphoribosylformylglycinamidine synthase activity
Gene Ontology
 GO:0003824 catalytic activity
Gene Ontology
 GO:0003674 molecular_function
Gene Ontology
 GO:0000166 nucleotide binding
Eggnog Protein
 EP:purL
Eggnog Ortholog
 EO:COG0046
Eggnog Description
 ED:Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL
EC Number
 EC:6.3.5.3 phosphoribosylformylglycinamidine synthase; phosphoribosylformylglycinamidine synthetase; formylglycinamide ribonucleotide amidotransferase; phosphoribosylformylglycineamidine synthetase; FGAM synthetase; FGAR amidotransferase; 5'-phosphoribosylformylglycinamide:L-glutamine amido-ligase (ADP-forming); 2-N-formyl-1-N-(5-phospho-D-ribosyl)glycinamide:L-glutamine amido-ligase (ADP-forming) kornec00230kornec01100kornec01110

Occurs in the following pathway maps:

Pathway Description
kornec00230 Purine metabolism
kornec01100 Metabolic pathways
kornec01110 Biosynthesis of secondary metabolites

Sequences

Nucleotide sequence (GC-content: 59.1 %):

ATGAAGCAAGCATTAACGCCAGAACAAATTAAGGAACAAAAGCCTTACTTGGACTGGAGCCTAAGCGAGCGCGAATACGACTACATTTCAGAAAAACTCCTGGGCCGGTTGCCTAACTTCACCGAAACCGGGCTCTTCTCCGCCATGTGGAGTGAACACTGTTCCTACAAGAAGTCCAAGCCGGTCTTGCGCTTGTTCCCCAACCAAAACGACCGGGTCCTCCAGGGGCCTGGTGAAGGGGCCGGGGTCGTTGACATCGATGACGGCCAAGCGGTGGTCTTCAAGGCCGAAAGCCACAACCACCCGTCGACGGTCGAACCTTACCAGGGGGCCGCAACCGGGGTCGGGGGGATCTTGCGTGATATCTTCTCGATGGGGGCACGGCCAATCGCCAGCCTCGATTCCCTGCACTTCGGCGAACTCGACGACCCGAAGATGCAAATGAAGGTCGCTGGTACCGTTAAGGGGATTGGGGATTACGGTAACTGCATGGGGATCCCAACCGTGGCCGGGGAAACGACCTTTGACCCGTGCTACTCGGGCAACCTCTTAGTCAACGCCATGAGCGTCGGCTTGATGGACCAAAAGGACATCCAAAAGGGGAAGGCCGCCGGGATCGGCAACGCCGTGATGTACGTCGGGGCCAAGACTGGTCGTGACGGGATCCACGGGGCAACCTTTGCCTCGGCCGACTTCTCCGACGAAAACGCCACCCAGCGGTCCGCCGTGCAAGTCGGGGACCCCTTCATGGAAAAGCTGCTCTTGGAAGCCTGCTTGGAATTGATTCAAAACCACCCCGACTGGTTAGTGGGGATCCAGGACATGGGGGCGGCCGGGATCGTGTCCTCGTCCGCCGAAATGGCGAGCGAAGGGGATGCGGGGATGGACCTCGACCTCGACCTCGTTCCGCAACGCGAACCGAACATGTCGGCCTACGAAATCATGCTGTCCGAGTCCCAAGAACGGATGCTGCTGTGCGTCAACAAGGGCCACGAAGACGACGTCAAAAAGATCTTTGACTTCTACGGCCTGGAAGCCGTCACGATCGGCCGGATCACGGAGGGGCGCCAGTACGTCTTGCACCACGACGGGCAAGTCGTTTGTGACATCCCGGTTTCCAGCTTAACCGACGACGTCTTAGAAGAAACCTCCGAAGAACAAAAGCCGGCCCGGATTTTGCAGGCCGAACAAGAGGCTAACTGGCAACCGGAAGTAACAGACGCCGGGGCGACCCTGGAACAACTGCTCCAACAGTCGACGATCGCCGACAAGTCAATCTTCTTTGAACAATACGACTCGATGGTTCGGACCTCGACGGTGGTCGGCCCCGGGTCTGACGCCGGGGTCCTGCGGGTCCGCGGCACCAAGAAGGGCCTGGCGATGACGACCGACGGCAACGGCCGCTTCGTGTACCTCAACCCGGAAGTTGGGGGCAAGATGGCCCTGGCCGAAGCGGCGGCTAACATCGTCGCCACTGGGGCCCTGCCACTGGCGATCACCGACTGCTTGAACTACGGGGACCCCAACGACCCAGAAATCTTCTGGGAGCTCCACCAATCAGTGTCCGGGATGGCCGAAGCCTGCCGGGTCTTTGACACCCCGGTCATTTCCGGGAACGTTTCCTTGTACAACGAAAATAACGGCCAGGCCATTCACCCCACCCCAATGGTCGGGATGGTCGGCCTGATCAAGGACTTAAAGAACCTGGTTAAGATGGCGGCCCAAGCAGCCGGCGACCAAGTCTACCTGCTGGGCCAAACCGGTGACGACTACGCCGGTAGCGAACTGCAAAAGATGCTGACCGGCGACATCGCCGGGACGGTGGCCGACTTCGACTTAAACCACGTCCACGCCATGATGCAAACCCTGCTGTCCCTAATGGAAGACGGCAAGGTAGCGTCCGCGCACGACCTGGCCGAAGGGGGCCTGGGGGTCGCCCTAGCCGAAACGCTCTTCAAGACCGATCTGGGGATGAACCTCAAGCTCGACTTAACCAAGAACCAACTCTTTAGTGAAACCGCTGGACGCTTTGTGGTGACCGTCAAAAAAGAAGACGCTGCGGCCTTTGAGGCGGCCCTGGGCGACGACGCCACGCTGATTGGGGAAGTTACCAACAGCCACTGGCTCCAGGTACGCCTGGCGGACGGCAACCTGAACAAAAACGTTGCCGACTTAGAACAATTGTGGAAGGAGGCCATTCCGTGCCAGCTGAAATCAAGGGATTAA

Protein sequence:

MKQALTPEQIKEQKPYLDWSLSEREYDYISEKLLGRLPNFTETGLFSAMWSEHCSYKKSKPVLRLFPNQNDRVLQGPGEGAGVVDIDDGQAVVFKAESHNHPSTVEPYQGAATGVGGILRDIFSMGARPIASLDSLHFGELDDPKMQMKVAGTVKGIGDYGNCMGIPTVAGETTFDPCYSGNLLVNAMSVGLMDQKDIQKGKAAGIGNAVMYVGAKTGRDGIHGATFASADFSDENATQRSAVQVGDPFMEKLLLEACLELIQNHPDWLVGIQDMGAAGIVSSSAEMASEGDAGMDLDLDLVPQREPNMSAYEIMLSESQERMLLCVNKGHEDDVKKIFDFYGLEAVTIGRITEGRQYVLHHDGQVVCDIPVSSLTDDVLEETSEEQKPARILQAEQEANWQPEVTDAGATLEQLLQQSTIADKSIFFEQYDSMVRTSTVVGPGSDAGVLRVRGTKKGLAMTTDGNGRFVYLNPEVGGKMALAEAAANIVATGALPLAITDCLNYGDPNDPEIFWELHQSVSGMAEACRVFDTPVISGNVSLYNENNGQAIHPTPMVGMVGLIKDLKNLVKMAAQAAGDQVYLLGQTGDDYAGSELQKMLTGDIAGTVADFDLNHVHAMMQTLLSLMEDGKVASAHDLAEGGLGVALAETLFKTDLGMNLKLDLTKNQLFSETAGRFVVTVKKEDAAAFEAALGDDATLIGEVTNSHWLQVRLADGNLNKNVADLEQLWKEAIPCQLKSRD

GenBank Info

gene - purL
locus_tag - FAM19471-i1-1.1_001330
EC_number - 6.3.5.3
inference - COORDINATES: similar to AA sequence:RefSeq:WP_013439864.1
note - Derived by automated computational analysis using gene prediction method: Protein Homology.
codon_start - 1
transl_table - 11
product - phosphoribosylformylglycinamidine synthase subunit PurL
protein_id - extdb:FAM19471-i1-1.1_001330

Gene Locus

Located on scaffold FAM19471-i1-1_scf27

FAM19471-i1-1.1_001329
FAM19471-i1-1.1_001331