update range to 10000 bp
locus tag: FAM19471-i1-1.1_001515 genome: FAM19471-i1-1.1
species: Limosilactobacillus fermentum taxid: 1613

Annotations

Type Name Description Pathways
Gene Product
 tRNA lysidine(34) synthetase TilS
Gene Code
 tilS
KEGG reaction
 R09597 L-lysine:[tRNA(Ile2)]-cytidine34 ligase (AMP-forming); [tRNA(Ile2)]-cytidine34 + L-Lysine + ATP <=> [tRNA(Ile2)]-lysidine34 + AMP + Diphosphate + H2O
KEGG reaction
 R02142 XMP:pyrophosphate phosphoribosyltransferase; Xanthosine 5'-phosphate + Diphosphate <=> Xanthine + 5-Phospho-alpha-D-ribose 1-diphosphate kornec00230kornec01100kornec01110
KEGG reaction
 R01229 GMP:diphosphate 5-phospho-alpha-D-ribosyltransferase; GMP + Diphosphate <=> Guanine + 5-Phospho-alpha-D-ribose 1-diphosphate kornec00230kornec01100
KEGG reaction
 R01132 IMP:diphosphate phospho-D-ribosyltransferase; IMP + Diphosphate <=> Hypoxanthine + 5-Phospho-alpha-D-ribose 1-diphosphate kornec00230kornec01100
Ortholog
 N0.HOG0001273 tRNA lysidine(34) synthetase TilS
KEGG gene
 K15780 tilS-hprT; bifunctional protein TilS/HprT [EC:6.3.4.19 2.4.2.8] kornec00230kornec01100kornec01110
KEGG gene
 K04075 tilS, mesJ; tRNA(Ile)-lysidine synthase [EC:6.3.4.19]
Eggnog Protein
 EP:tilS
Eggnog Ortholog
 EO:COG0037
Eggnog Description
 ED:Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU
EC Number
 EC:6.3.4.19 tRNAIle-lysidine synthase; TilS; mesJ (gene name); yacA (gene name); isoleucine-specific transfer ribonucleate lysidine synthetase; tRNAIle-lysidine synthetase
EC Number
 EC:2.4.2.8 hypoxanthine phosphoribosyltransferase; IMP pyrophosphorylase; transphosphoribosidase; hypoxanthine---guanine phosphoribosyltransferase; guanine phosphoribosyltransferase; GPRT; HPRT; guanosine 5'-phosphate pyrophosphorylase; IMP-GMP pyrophosphorylase; HGPRTase; 6-hydroxypurine phosphoribosyltransferase; 6-mercaptopurine phosphoribosyltransferase; GMP pyrophosphorylase; guanine-hypoxanthine phosphoribosyltransferase; guanosine phosphoribosyltransferase; guanylate pyrophosphorylase; guanylic pyrophosphorylase; inosinate pyrophosphorylase; inosine 5'-phosphate pyrophosphorylase; inosinic acid pyrophosphorylase; inosinic pyrophosphorylase; purine-6-thiol phosphoribosyltransferase kornec00230kornec00983kornec01100kornec01110

Occurs in the following pathway maps:

Pathway Description
kornec00230 Purine metabolism
kornec00983 Drug metabolism - other enzymes
kornec01100 Metabolic pathways
kornec01110 Biosynthesis of secondary metabolites

Sequences

Nucleotide sequence (GC-content: 56.8 %):

ATGAAAGACCCTAAATTAACGATGGAGCGGTCCCCCCTGGGGAGCCGCTTTTTTTCGTCAAGCAACACCTTAGTCGTGGCCGTCTCAACCGGGGTGGACTCAATGGTGCTCTTAGACCTGCTCCAAAACTTACCTAAGTTAAGGCGTCCCCGTTTAATCGTTGCCCACGTTAACCACCACTTGCGTACCCAAAGCACCGCAGAAGAAGCCTTTTTACGTGCGTATTGCCGGCAACGAAACCTTCCCCTCGAAGTGGCCCAGTGGGCGGTCGCAGAGCACCCAGCGAAGGGAATTGAAGCGGCGGCCCGCCGGTTTCGCTACTCCTTTTTTGCCACGGTGATGGACAAATACCAAGCCCAGGCGGTGGCAACGGCGCACCACCAAGACGACCTGGCGGAAACGGTCCTGATGAAGTTAATTCGTGGTGGTCGGATCGAACAATTAGGAGCGCTGAGATGGCAGCGCCCCTTTGCCAGTGGCGCACTAATTCGCCCTCTACTCGCGCTTAACAAACAAGACCTCCGCCGCTATGCTAAGGACCATCAGTTGACCTGGTTTGAAGATGAAACAAACCGTGATCTAACCCCGTTTCGCAACCGGGTTCGGCACCGCTACCTTCCAAGCCTCAGCGTTGAAAACCCCCGGCTCGTCACAGCGCTGGCTAGTGACGCCGAGCAAATCAATGACCTCTTGGGCTTGGCTAACGAACGACTAGGGGAACTCGATGCCACCCTGCGGACCGAACGGGGCTTTTCGTTGGCCGCCTTTCGTCGGTTGAGTGAAAGTACCCAACGTTCCTACCTCCGCTACTACCTAACCAAACGGGGCGTTGCAACGATTAAAGAAGAACAGCTTCACCAACTGGTAACCGGGTTAGGCGACCAAACCAGGCCGCCCCAGAGCTTCCAACTGCCCAATCAGTGGTGCTTGGTTAAGGGCTACCGGGAGGCCTTCCTTAAAAAAGCCGGGCAAATGGCCCAGGAAGGCGAATCGGAGGGGGAGTTTGTGGTAGAATTGGGCCAGTGGTATCGTCTCCGGGCGGGGGGGGCTTTTTCAGTCACCCGCCAGCCAGACCAAGGGTCAGTGACCACGGTTTGGTTGACTAACAACCAGTTTCCCCTGACTTGCCGTCCCGTTCAAGCTGGTGACCGGCTATTGTTAAAGGGGGGCACACACCAACGGGTTCGGCGGGTCTTGATCGACCAAAAGGTTCCCCGTGACTTACGGGCGAACCAACGGGTGATCGTCGACGCCTTAGGCCAAGTGGTGTGGTTAGTCGGGGTGAAGGTGGCTTGGTTTGACCGCCGACCAGGGGCGCTACCGGTAGCGTTATGCCAACGAGAACAATGA

Protein sequence:

MKDPKLTMERSPLGSRFFSSSNTLVVAVSTGVDSMVLLDLLQNLPKLRRPRLIVAHVNHHLRTQSTAEEAFLRAYCRQRNLPLEVAQWAVAEHPAKGIEAAARRFRYSFFATVMDKYQAQAVATAHHQDDLAETVLMKLIRGGRIEQLGALRWQRPFASGALIRPLLALNKQDLRRYAKDHQLTWFEDETNRDLTPFRNRVRHRYLPSLSVENPRLVTALASDAEQINDLLGLANERLGELDATLRTERGFSLAAFRRLSESTQRSYLRYYLTKRGVATIKEEQLHQLVTGLGDQTRPPQSFQLPNQWCLVKGYREAFLKKAGQMAQEGESEGEFVVELGQWYRLRAGGAFSVTRQPDQGSVTTVWLTNNQFPLTCRPVQAGDRLLLKGGTHQRVRRVLIDQKVPRDLRANQRVIVDALGQVVWLVGVKVAWFDRRPGALPVALCQREQ

GenBank Info

gene - tilS
locus_tag - FAM19471-i1-1.1_001515
EC_number - 6.3.4.19
inference - COORDINATES: similar to AA sequence:RefSeq:WP_003686489.1
note - Derived by automated computational analysis using gene prediction method: Protein Homology.
codon_start - 1
transl_table - 11
product - tRNA lysidine(34) synthetase TilS
protein_id - extdb:FAM19471-i1-1.1_001515

Gene Locus

Located on scaffold FAM19471-i1-1_scf34

FAM19471-i1-1.1_001514
FAM19471-i1-1.1_001516